Chronological expression of Wnt target genes Ccnd1, Myc, Cdkn1a, Tfrc, Plf1 and Ramp3 |
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Authors: | Sonja Rö hrs,Nadine Kutzner,Thomas Grunwald,Oliver Mü ller |
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Affiliation: | a Max-Planck-Institut für molekulare Physiologie, Dortmund, Germany b Abteilung für molekulare und medizinische Virologie, Ruhr-Universität Bochum, Germany |
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Abstract: | The canonical Wnt pathway regulates several biological processes including development, cell growth and proliferation via consecutive gene regulation. A high number of target genes of the Wnt pathway has been identified, but the chronological order of target gene expression is still elusive. This order is supposed to be crucial for the controlled course of events downstream of the activated Wnt pathway. Here we present the expression chronologies of the target genes Ccnd1 (encoding for cyclin D1), Myc (c-Myc), Cdkn1a (p21CIP1/WAF1), Tfrc (Transferrin receptor 1), Plf1 (Proliferin-1) and Ramp3 (Receptor activity-modifying protein 3) in C57MG cells after stimulation with Wnt-3a. We discriminated between immediate (below 1 h), early (between 1 and 6 h), intermediate (between 6 and 12 h) and late (after 12 h) targets. According to this classification Myc and Tfrc belong to the immediate target genes; Ccnd1, Plf1 and Ramp3 are early target genes and Cdkn1a is an intermediate target gene. |
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Keywords: | Wnt signalling pathway Wnt target genes β-catenin Cyclin D1 c-Myc p21CIP1/WAF1 Transferrin receptor 1 Proliferin-1 Ramp3 |
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