Pyrene-modified guanosine as fluorescent probe for DNA modulated by charge transfer |
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Authors: | Wanninger-Weiss Claudia Valis Linda Wagenknecht Hans-Achim |
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Affiliation: | University of Regensburg, Institute for Organic Chemistry, Universit?tstr. 31, D-93053 Regensburg, Germany. |
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Abstract: | 8-(Pyren-1-yl)-2'-deoxyguanosine (Py-G) was incorporated synthetically as a modified DNA base and optical probe into oligonucleotides. A variety of Py-G-modified DNA duplexes have been investigated by methods of optical spectroscopy. The DNA duplex hybridization can be observed by both fluorescence and absorption spectroscopy since the Py-G group exhibits altered properties in single strands versus double strands for both spectroscopy methods. The fluorescence enhancement upon DNA hybridization can be improved significantly by the presence of 7-deazaguanin as an additional modification and charge acceptor three bases away from the Py-G modification site. Moreover, Py-G in DNA can be applied as a photoinducable donor for charge transfer processes when indol is present as an artificial DNA base and charge acceptor. Correctly base-paired duplexes can be discriminated from mismatched ones by comparison of their fluorescence quenching. |
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