Synthesis of the growth hormone-regulated rat liver anti-protease GHR-P63 is inhibited by acute inflammation

Synthesis of the growth hormone-regulated anti-protease GHR-P63 by rat hepatocytes was strongly reduced during acute inflammation. This decrease was detected 8 h after the onset of inflammation and reached a maximum after 24 h. A decrease in the GHR-P63 mRNA level measured by in vitro translation and by hybridization mainly accounted for the alteration of GHR-P63 synthesis. Besides this major pretranslational mechanism, inflammation also interfered with GHR-P63 synthesis at a posttranslational level. This was indicated by the production of abnormal immunoprecipitable species at early stages of the acute-phase response.