Plant regeneration from shoot callus of rosewood (Dalbergia latifolia Roxb.) |
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Authors: | G. Lakshmi Sita S. Chattopadhyay D. H. Tejavathi |
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Affiliation: | (1) Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012 Bangalore, India;(2) Botany Department, Bangalore University Jnanabharathi, 560 056 Bangalore, India |
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Abstract: | In vitro propagation of trees using cell, tissue and organ culture is a fast emerging area. We report here the clonal propagation of Indian rosewood (Dalbergia latifolia Roxb.) from shoot callus cultures of 5 year old trees. Bud regeneration was obtained on MS media supplemented with BA and NAA. About 35% of the cultures showed organogenesis. Shoots measuring about 3–5 cm can be excised and rooted in White's medium supplemented with 1–2 mg/L IAA. Rooted plants were successfully established in soil.Abbreviations BA Benzyladenine - CM Coconut milk - 2,4-D 2,4 dichlorophenoxyacetic acid - IAA Indoleacetic acid - IBA Indolebutyric acid - K Kinetin - NAA Naphthaleneacetic acid - PVP-360 Polyvinyl pyrrolidone |
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