Improved technique for accurate and convenient assay of biological reactions liberatingCO2 |
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| Authors: | CH Sissons |
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| Institution: | Section of Biochemistry and Molecular Biology, 270 Clark Hall, Cornell University, Ithaca, New York 14853 USA |
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| Abstract: | Use of a convenient and inexpensive apparatus for trapping14CO2 from biological reactions in small volumes is described. Alternative scintillants for estimating14CO2 trapped in KOH are compared, toluene methoxyethanol (2:1) based scintillants and Instagel (TM-Packard) were efficient and more stable than the dioxane/Cab-O-Sil or toluene/Triton X-100 mixtures usually used. The usefulness of the technique is illustrated with microassays of OMP decarboxylase (EC 4.1.1.23), OMP pyrophosphorylase (EC 2.4.2.10) in the fission yeastSchizosaccharomyces pombe. |
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| Keywords: | To whom correspondence and reprint requests should be addressed |
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