Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India |
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Authors: | Parashar Deepti Chauhan D S Sharma V D Chauhan Aradhana Chauhan S V S Katoch V M |
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Affiliation: | Central JALMA Institute for Leprosy and Other Mycobacterial Diseases, Indian Council of Medical Research, Tajganj, Agra 28201, India. |
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Abstract: | For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria. |
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