BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3' |
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Authors: | Degtyarev S K Belichenko O A Lebedeva N A Dedkov V S Abdurashitov M A |
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Affiliation: | SibEnzyme, 9 Ak.Timakov Str., Novosibirsk 630117, Russia. |
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Abstract: | The recognition sequence and cleavage positions of a new restriction endonuclease BTR:I isolated from Bacillus stearothermophilus SE-U62 have been determined. BTR:I belongs to a rare type IIQ of restriction endonucleases, which recognise non-palindromic nucleotide sequences and cleave DNA symmetrically within them. |
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