| 中国红豆杉细胞悬浮体系营养条件的优化 |
| |
| 引用本文： | 梅兴国,熊乾斌,余斐. 中国红豆杉细胞悬浮体系营养条件的优化[J]. 生物技术, 2001, 11(5): 5-7 |
| |
| 作者姓名： | 梅兴国 熊乾斌 余斐 |
| |
| 作者单位： | 华中科技大学生命科学与技术学院 |
| |
| 基金项目： | 国家科委"八五”、"九五”重点科技攻关资助项目(85-08-06-02,96-C02-03-01) |
| |
| 摘 要： | 采用均匀设计的试验方法,对中国红豆杉细胞悬浮体系培养条件进行优化,获得了关于细胞生长与培养基内碳源,氮源,磷浓度的回归方程,表明碳氮是影响细胞生长的关键因素。采用中心组合实验设计对培养基成分继续优化,并通过回归分析,结果表明,较优的培养基为碳源33．9g/L,氮源58．2mmol/L,磷浓度为5mmol/L,此工艺在15L反应器中,也取得了良好效果。
|
| 关 键 词： | 均匀设计 中心组合实验设计 细胞悬浮培养 数学模型 红豆杉 营养条件 优化 |
| 文章编号： | 1004-311(2001)05-0005-03 |
| 修稿时间： | 2001-03-15 |
Optimization on culture conditions in cell suspensions of Taxus chinensis |
| |
| MEI Xing guo,XIONG Qian bin,YU Fei. Optimization on culture conditions in cell suspensions of Taxus chinensis[J]. Biotechnology, 2001, 11(5): 5-7 |
| |
| Authors: | MEI Xing guo XIONG Qian bin YU Fei |
| |
| Abstract: | In order to optimize the culture contions in cell suspensions of Taxus chinensis,uniform design is employed to test the improvement of medium components.The results showed that saccharrose.nitrogen were the key factors affecting the biology weight.On this basis,by means of central combination design,the mathematical model on the factors and the growth was set up,and the parameters were defined:saccharrose 33.9g/L,nitrogen 58.2mmol/L.phosphor 5mmol/L.and we also made great improvement in 15L biology rectors. |
| |
| Keywords: | uniform design central combination design suspension culture mathematical model |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
