贵州核桃核心种质筛选与构建研究北大核心CSCD

为了更好地构建贵州核桃核心种质,该研究以245份贵州核桃种质资源为试材,利用数量性状的遗传变异数据,对其构建方法进行了探索;采用随机取样策略、偏离度取样策略和位点优先取样策略,结合8个取样比例(5%、10%、15%、20%、25%、30%、40%和50%),进行多次聚类构建核心种质;将核心种质与原种质和保留种质进行t检验比较来评价核心种质,并用主成分分析法和表型性状对核心种质进行确认。结果表明:(1)选取偏离度取样策略,总体取样比例为50%,构建出131份贵州核桃核心种质资源。(2)对种质资源核心库13个数量性状进行主成分分析,其累计贡献率达到76.48%,主成分图中核桃核心种质与原种质的分布较为相似,表明核心种质有效地保存了核桃原种质的遗传结构,并有效避免了种质的冗余。(3)在欧氏距离结合组间联接法的聚类条件下,偏离度取样策略是构建贵州核桃核心种质的最佳方法。

Study on Screening and Construction of Guizhou Walnut Core Collection

To better construct walnut core germplasm, this paper uses 245 walnut germplasm resources as test materials and uses the genetic variation data of quantitative traits to explore its construction methods. We used random sampling strategy, deviation degree sampling strategy, and site priority sampling strategy, combined with 8 gradients (5%, 10%, 15%, 20%, 25%, 30%, 40% and 50%). The sampling ratio of the core collection was constructed by clustering multiple times. The core collection was compared with the original collection and the reserved collection by t test. The core collection was evaluated, and the core collection was confirmed by principal component analysis and phenotypic traits. The results showed that: (1) the deviation degree sampling strategy was selected, the overall sampling ratio was 50%, and 131 core germplasm resources of walnut group Guizhou walnut plants were constructed. (2) The principal component analysis of 13 quantitative traits in the core pool of germplasm resources was carried out, and the cumulative contribution rate reached more than 76.48%. The distribution of walnut core germplasm and original germplasm in the principal component diagram was similar. In this way, the genetic structure of the original walnut germplasm is effectively preserved, and the redundancy of the germplasm is effectively avoided. (3) Under the clustering conditions of Euclidean distance combined with the inter group connection method, the deviation degree sampling strategy is the best method to construct walnut core collection.