Slo2 sodium-activated K+ channels bind to the PDZ domain of PSD-95 |
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Authors: | Uchino Shigeo Wada Hidenori Honda Shizuyo Hirasawa Takae Yanai Shigeki Nakamura Yasuko Ondo Yumiko Kohsaka Shinichi |
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Institution: | Department of Neurochemistry, National Institute of Neuroscience, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan. |
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Abstract: | Slo2 channels are a type of sodium-activated K+ channels and possess a typical PDZ binding motif at the carboxy-terminal end. Thus, we investigated whether Slo2 channels bind to PSD-95, because it is well known that other types of K+ channels, voltage-gated and inward rectifier K+ channels, bind to PSD-95 via the PDZ binding motif and are involved in excitatory synaptic transmission. By using an extract prepared from cultured neocortical neurons, we demonstrated a biochemical interaction between mSlo2 channels and PSD-95, and a mutational analysis revealed that mSlo2 channels bound to the first PDZ domain of PSD-95 via the PDZ binding motif. To investigate the expression of mSlo2 protein in primary neocortical neurons, we raised anti-mSlo2 channel antibody and immunostained neocortical neurons. The immunocytochemical study showed that mSlo2 channels partly colocalized with PSD-95 in mouse neocortical neurons. |
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Keywords: | Sodium-activated K+ channel Slo2 channel PSD-95 PDZ domain Excitatory neuron |
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