Characterization ofEntamoeba histolytica-induced dephosphorylation in Jurkat cells |
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Authors: | J E Teixeira B J Mann |
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Institution: | (1) Department of Internal Medicine, University of Virginia, 22908 Charlottesville, VA, USA;(2) Department of Microbiology, University of Virginia, 22908 Charlottesville, VA, USA |
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Abstract: | Entamoeba histolytica killing of host cells is contact dependent and mediated by a Gal/GalNAc lectin. Upon contact with amoeba a rapid and extensive
dephosphorylation of tyrosine phosphorylated host cell proteins is observed. This effect is mediated by the Gal/GalNAc lectin.
However, it requires intact cells, as purified lectin failed to induce dephosphorylation in Jurkat cells. The nonpathogenic,
but morphologically identical amoeba,Entamoeba moshkovskii also did not induce dephosphorylation in target cells. Treatment of Jurkat cells with phosphotyrosine phosphatase inhibitors
has shown that a host phosphatase is responsible for dephosphorylation. However, it was found that the CD45 phosphotase was
not necessary for dephosphorylation of host cell proteins. |
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Keywords: | Apoptosis CD45 Entamoeba moshkovskii Jurkat cells |
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