Retinol-induced elevation of ornithine decarboxylase activity in cultured rat Sertoli cells is attenuated by free radical scavenger and by iron chelator |
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Authors: | Klamt Fábio Dal-Pizzol Felipe Ribeiro Nede Carlos Barnard Elena Aida Silveira Benfato Mara Fonseca Moreira José Cláudio |
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Affiliation: | (1) Laboratório de Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600, Porto Alegre, RS, Brazil;(2) Departamento de Biofísica, Universidade Federal do Rio Grande do Sul, Campus do Vale, Porto Alegre, RS, Brazil |
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Abstract: | We investigated retinol effects in ornithine decarboxylase activity in Sertoli cells. We also tested the hypothesis that free radical scavengers and iron chelators may attenuate the effect of retinol. Sertoli cells isolated from 15-day-old Wistar rats were previously cultured for 48 h and then treated with retinol by 24 h with or without mannitol (1 mM) or 1,10 phenanthroline (100 M). We measured ornithine decarboxylase and catalase activities and malondialdehyde concentrations in response to retinol treatment. In response to 7 M retinol treatment ornithine decarboxylase activity increased 30%. Retinol-induced ornithine decarboxylase activity was significantly decreased by addition of free radical scavenger (mannitol) or iron chelator (1,10 phenanthroline). In addition the same effect was observed in catalase increased activity and in malondialdehyde concentrations. These results suggest that retinol treatment induced ornithine decarboxylase and catalase activity and increased malondialdehyde concentration. These effects appear to be mediate by ROS. |
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Keywords: | retinol ornithine decarboxylase reactive oxygen species iron chelator catalase |
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