Efficient transformation ofKlebsiella oxytoca by electroporation |
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| Authors: | Ki Jun Jeong Hyun Sook Lee Sang Yup Lee Yong Keun Chang |
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| Institution: | (1) Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kusong-dong, Yusong gu, 305-701 Taejon, Korea |
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| Abstract: | A protocol for the transformation ofKlebsiella oxytoca by electroporation was developed. Preparation of competent cells at early exponential phase was most critical to obtain a
high transformation efficiency. The highest efficiency of 1.6 × 106 transformants per μg DNA (pBR 322) could be obtained by electroporation ofK. oxytoca cells prepared at the OD600 of 0.2 with 1.25 μg DNA at the filed strength of 2.5 kV, the parallel resistance of 200 Ω and capacitance of 25 μF. |
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| Keywords: | Klebsiella oxytoca electroporation efficiency |
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