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Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production |
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| Authors: | Matthew N. Henry Michael A. MacDonald Camila A. Orellana Peter P. Gray Marianne Gillard Kym Baker Lars K. Nielsen Esteban Marcellin Stephen Mahler Verónica S. Martínez |
| Affiliation: | 1. Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia;2. ARC Training Centre for Biopharmaceutical Innovation (CBI), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia;3. ARC Training Centre for Biopharmaceutical Innovation (CBI), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia Patheon Biologics—A Part of Thermo Fisher Scientific, Brisbane, Queensland, Australia;4. Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia ARC Training Centre for Biopharmaceutical Innovation (CBI), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia Metabolomics Australia, The University of Queensland, Brisbane, Queensland, Australia |
| Abstract: | Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells. |
| Keywords: | apoptosis biopharmaceutical production CHO cell line engineering programmed cell death |