| 一株龙葵内生细菌SDE06去除Cd2+的实验 |
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| 引用本文： | 曹 喆,罗胜联,曾光明,肖 潇,万 勇,苏 峰.一株龙葵内生细菌SDE06去除Cd2+的实验[J].微生物学通报,2009,36(3):0328-0333. |
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| 作者姓名： | 曹 喆 罗胜联 曾光明 肖 潇 万 勇 苏 峰 |
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| 作者单位： | 湖南大学环境科学与工程学院,湖南,长沙,410082 |
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| 基金项目： | 国家自然科学基金重点项目(No. 50830301); 湖南省重大专项(No. 2008SK1002) |
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| 摘 要： | 植物内生菌广泛存在于各种植物中, 对宿主的生命活动产生了各种影响。本研究通过对重金属镉(Cd)超累积植物龙葵(Solanum nigrum L.)内生菌优势种进行分离纯化, 并用含Cd2+培养基初步筛选, 得到7株有抗性的菌株, 分别命名为SDE01-07,其中SDE06在Cd2+浓度为80 mg/L的条件下仍能生长。经鉴定此株菌属芽孢杆菌属(Bacillus sp.)。对SDE06在不同条件下去除Cd2+的情况进行研究, 结果表明：正交实验得最佳实验条件为培养时间36 h, pH 6.0, 温度37°C
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| 关 键 词： | 超累积植物 龙葵 内生菌 去除率 Cd2+ |
Removal of Cd2+ by an Endophytic Bacteria SDE06 Obtained from Solanum Nigrum L. |
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| CAO Zhe,LUO Sheng-Lian,ZENG Guang-Ming,XIAO Xiao,WAN Yong and SU Feng.Removal of Cd2+ by an Endophytic Bacteria SDE06 Obtained from Solanum Nigrum L.[J].Microbiology,2009,36(3):0328-0333. |
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| Authors: | CAO Zhe LUO Sheng-Lian ZENG Guang-Ming XIAO Xiao WAN Yong and SU Feng |
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| Institution: | College of Environmental Science and Engineering Hunan University, Changsha, Hunan 410082, China;College of Environmental Science and Engineering Hunan University, Changsha, Hunan 410082, China;College of Environmental Science and Engineering Hunan University, Changsha, Hunan 410082, China;College of Environmental Science and Engineering Hunan University, Changsha, Hunan 410082, China;College of Environmental Science and Engineering Hunan University, Changsha, Hunan 410082, China;College of Environmental Science and Engineering Hunan University, Changsha, Hunan 410082, China |
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| Abstract: | The endophytic microorganisms found widely in many kinds of plants mediate various effects to theirs hosts. In this study, seven different dominant endophytes (SDE01 to 07) isolated from a Hyperaccumulator-Solanum nigrum L. were resistant to Cd2+, and the strain SDE06 survived even in the medium containing 80 mg/L of Cd2+. Bacteria strain SDE06 was identified as Bacillus sp.. The removal of Cd2+ of SDE06 in different conditions were studied. Under the optimal conditions, the incubating time was 36 h, the solution pH 6.0, the temperature was 37°C and the Cd2+ concentration of medium was 20 mg/L, the highest removal rate was up to 80.2% at this condition. |
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| Keywords: | Hyperaccumulator Solanum nigrum L Endophytes Removal rate Cd2+ |
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