Bovine serum sialic acid: Age-related changes in type and content |
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| Institution: | 1. Department of Clinical Epidemiology, College of Medicine, University of the Philippines Manila, Manila, Philippines;2. Department of Neurosciences, College of Medicine and Philippine General Hospital, University of the Philippines Manila, Manila, Philippines;3. Division of Neurology, Department of Medicine, St. Michael’s Hospital, University of Toronto, ON M5B 1W8, Canada;4. Division of Rheumatology, Department of Medicine, University of Toronto and University Health Network Toronto Western Hospital, Toronto, ON M5T 2S8, Canada |
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| Abstract: | - 1.1. The sialic acid content of newborn calf serum (4.8 μmol/ml) is approx. 3-fold higher than that of mature animals (1.4 μmol/ml) and decreases to 2.4 μmol/ml at 20 days of age. Colostrum-fed and colostrum-deprived calves have similar levels of sialic acid from birth to 14 days of age.
- 2.2. The high level of sialic acid in newborn calf serum is due predominantly to N-acetylneuraminic acid, since this sialic acid accounts for 93% of the total and since <5% of the sialic acid is O-acetylated.
- 3.3. Comparison of day 0 and day 20 serum by gel filtration and by SDS polyacrylamide gel electrophoresis demonstrates that the increase in sialic acid is associated with increased production and/or sialylation of components with MW of 45–60 kDa.
- 4.4. A high percentage (64%) of the sialic acid in newborn calf serum is detected with the lipid-linked sialic acid assay, relative to 20 day old (25%) or mature (18%) animals.
- 5.5. This indicates that the glycoproteins of newborn calf serum are more efficiently extracted under the conditions of this assay than glycoproteins of mature serum.
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