Identification and quantification of C21 and C19 steroids in the haemolymph of Leptinotarsa decemlineata,a phytophagous insect |
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| Institution: | 1. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China;2. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;4. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;5. CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;6. Zhongke Tonhe (Shandong) Marine Technology Co., Ltd, Dongying 257200, China |
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| Abstract: | o-Pentafluorobenzyloxime (OPFB)-heptafluorobutyrylester (HFB)-derivatives were prepared from extracts of haemolymph from last instar larvae of Leptinotarsa decemlineata and subjected to negative ion chemical ionization capillary gas chromatography-mass spectrometry (NCI/GC-MS). Ten C21 and C19 steroids could be positively identified: testosterone, dehydroepiandrosterone, 5α-dihydrotestosterone, 11-ketotestosterone, 11β-hydroxytestosterone, androstenedione, progesterone, 17α-hydroxyprogesterone, pregnenolone and 17α,20β-dihydroprogesterone. No estrogens could be found in these larvae. Radioimmunoassay of chromatographed extracts of haemolymph taken from the larval and pupal stages showed fluctuations in testosterone (and 5α-dihydrotestosterone) titer. |
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