Biodegradation of lindane by a native bacterial consortium isolated from contaminated river sediment |
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Authors: | Silvia F. Pesce Daniel A. Wunderlin |
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Affiliation: | Deparmento Bioquímica Clínica, Facultad de Ciencias Químicas,Universidad Nacional de Córdoba, Pabellón Argentina, Ciudad Universitaria, Córdoba 5000, Argentina |
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Abstract: | The aerobic biodegradation of lindane (γ-hexachlorocyclohexane) by a consortium of acclimated bacteria from sediment at a polluted site on the Suquia River, Cordoba, Argentina, is reported. The bacteria were acclimated for 30 days under aerobic conditions, using a minimal culture medium containing lindane (0.034 mM) as sole carbon source. Growth of the bacterial consortium decreased at a lindane concentration of 1.03 mM and was totally inhibited at 2.41 mM. The consortium showed initial lindane degradation rates of 4.92×10−3, 11.0×10−3 and 34.8×10−3 mM h−1 when exposed to lindane concentrations of 0.069, 0.137 and 0.412 mM, respectively. Chloride concentration increased during aerobic biodegradation, indicating lindane mineralization. A metabolite identified as γ-2,3,4,5,6-pentachlorocyclohexene appeared during the first 24 h of biodegradation. Four different bacteria, identified as Sphingobacterium spiritivorum, Ochrobactrum anthropi, Bosea thiooxidans and Sphingomonas paucimobilis, were isolated. Pure strains of B. thiooxidans and S. paucimobilis degraded lindane after 3 days of aerobic incubation. This is the first report of lindane biodegradation by B. thiooxidans. |
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Keywords: | Lindane Biodegradation Aerobic Bosea thiooxidans Sphingomonas paucimobilis |
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