Differentiation of Langerhans Cells from Interdigitating Cells Using CD1a and S-100 Protein Antibodies |
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Authors: | Masanori Shinzato Mikihiro Shamoto Satoru Hosokawa Chiyuki Kaneko Akido Osada Miyuki Shimizu Asako Yoshida |
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Institution: |
a Division of Pathological Cytology, Institute for Comprehensive Medical Science, Fujita Health University, Aichi, Japan |
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Abstract: | The present study shows that Langerhans cells can be differentiated from Interdigitating cells at the light microscopic level. Superficial lymph nodes and skin taken from necropsies and the lymph nodes of dermatopathic lymphadenopathy (DPL) were used for this experiment. Sections of lymph node and skin were embedded using the acetone, methyl benzoate and xylene (AMeX) method and dendritic cells were immunostained with anti S-100 protein antibody (S-100, and OKT-6 (CD1a) using the restaining method. Langerhans cells in the skin were positive for both CD1a and S-100. Dendritic cells positive for both CD1a and S-100, and dendritic cells positive for S-100, but not for CD1a were observed in superficial lymph nodes. In normal superficial lymph nodes, there were more interdigitating cells than Langerhans cells. The majority of the dendritic cells in the DPL were Langerhans cells. We conclude that the S-100 and CD1a positive cells are Langerhans cells, and the S-100 positive-CD1a negative cells are interdigitating cells. |
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Keywords: | Langerhans cell interdigitating cell Anti S-100 protein antibody CD1a |
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