Influence of triacontanol on somatic embryogenesis in <Emphasis Type="Italic">Coffea arabica</Emphasis> L. and <Emphasis Type="Italic">Coffea canephora</Emphasis> P. ex Fr. |
| |
| Authors: | P?Ciridhar E?P?Indu Email author" target="_blank">G?A?RavishankarEmail author A?Chandrasekar |
| |
| Institution: | (1) Plant Cell Biotechnology Department, Central Food Technological Research Institute, 570013 Mysore, India;(2) Food Microbiology Department, Central Food Technological Research Institute, 570013 Mysore, India |
| |
| Abstract: | Summary A highly reproducible method for regeneration of Coffea arabica and C. canephora plants via direct somatic embryogenesis from cultured leaf and stem segments of regenerated plants was developed. Embryogenesis
was influenced by the presence of triacontanol (TRIA) in the medium. TRIA incorporated at 4.55 and 11.38 μM in half-strength MS basal medium containing 1.1 μM 6-benzyladenine (BA) and 2.28 μM indole-3-acetic acid (IAA) induced direct somatic embryogenesis in both species. A maximum of 260±31.8 and 59.2±12.8 somatic
embryos per culture were induced from in vitro leaf explants of C. arabica and C. canephora, respectively. TRIA also induced embryo formation from in vitro stem segment callus tissues along with multiplication of primary embryos into secondary embryos. By using TRIA, it was possible
to obtain somatic embryogenesis in C. arabica and C. canephora. |
| |
| Keywords: | Coffea hypocotyls leaf somatic embryos triacontanol |
| 本文献已被 SpringerLink 等数据库收录！ |
