Production of higher levels of trigonelline by cell cultures of Trigonella foenum-graecum than by the differentiated plant |
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Authors: | S. S. Radwan C. K. Kokate |
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Affiliation: | (1) Institut für Biochemie und Technologie, H.P. Kaufmann-Institut der Bundesanstalt für Fettforschung, Piusallee 68, D-4400 Munster, Federal Republic of Germany;(2) Present address: Department of Botany, Faculty of Science, Ains Sham University, Cairo, Egypt;(3) Present address: Bombay College of Pharmacy, Kalina, Bombay-29, India |
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Abstract: | Callus cultures of Trigonella foenum-graecum contained 3 to 4 times more trigonelline than the seeds of this plant and 12 to 13 times more than the roots and shoots. Even higher levels of this alkaloid were produced by suspension cultures. This high productivity was maintained during successive subculturing of calli and cell suspensions for eight months. Thus, trigonelline is to be added to the group of the few metabolites whose synthesis in cell cultures exceeds its production in the differentiated plants. Media that had supported the growth of suspension cultures contained one third or more of the total alkaloid, whereas media of callus cultures contained about one tenth of this substance. Trigonelline accumulated in callus and suspension cultures with aging. Raising the level of nicotinic acid in the nutrient medium resulted in some increase of trigonelline production by the culture.Abbreviations 2.4 D 2.4-dichlorophenoxyacetic acid - IAA indoleacetic acid - IPA indolepropionic acid - NAA -naphthaleneacetic acid - GA Gibberellic acid - K kinetin |
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Keywords: | Callus cultures Hormonal shocks Nicotinic acid Suspension cultures Trigonella Trigonelline |
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