玫瑰茄悬浮细胞合成花青素的光效应研究

光照对悬浮培养的玫瑰茄细胞生物量无影响。随着光照强度增大，玫瑰茄细胞合成花青素的量增加，光照强度３１．０ｗ／ｍ２为饱和光照强度，超过该强度，玫瑰茄细胞合成花青素的量不再进一步增加；可见光中蓝光（４２０～５３０ｎｍ）是促进玫瑰茄细胞合成花青素最有效单色光，光强为３０．０ｗ／ｍ２，接种量为０．２ｇ湿细胞的５０ｍｌ培养液经１６ｄ培养，花青素产量为８．９７ｍｇ／５０ｍｌ，高出相同光照强度全色光下的６．５３ｍｇ／ｍｌ；黄光和绿光分别有一定的促进作用。当黑暗下的培养时间不超过８ｄ，后期经过不少于８ｄ的光照可以诱导出和全程光照相当的花青素产量，分别为６．６４和６．７２ｍｇ／５０ｍｌ（总培养时间不少于１６ｄ）。当黑暗下培养时间超过１２ｄ，由于营养成分消耗，光照延长，花青素产量也无法提高，添加１０ｍｌ新鲜培养基再进行光诱导，花青素产量可以提高（６．７５ｍｇ／５０ｍｌ）。

Effects of light irradiation on anthocyanin synthesis in roselle (Hibiscus sabdariffa L.) cells suspension culture

Light irradiation did not affect the biomass of roselle cell suspension. With t he irradiation intensified, the anthocyanin synthesis increased until 31. 0 w/m 2. The blue(with wavelength of 420-530nm) was the most positive effective monochro mic light through visible spectrum to anthocyanin formation in roselle cells, an d the red(more than 600nm), the least effective, with the pigment yields of 8.97 and 0.81mg/50ml respectively. Light irradiation was able to induce anthocyanin s ynthesis in dark precultured roselle cells, useless when the nutrients in suspe nsion was exhausted.