Influence of Mannosylation on Immunostimulating Activity of Adamant‐1‐yl Tripeptide |
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| Authors: | Rosana Ribić Lidija Habjanec Ruža Frkanec Branka Vranešić Srđanka Tomić |
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| Affiliation: | 1. Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR‐10000 Zagreb (phone: +385‐1‐4606402;2. fax: +385‐1‐4606401);3. Institute of Immunology, Rockfellerova 2, HR‐10000 Zagreb |
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| Abstract: | The mannosylated derivative of adamant‐1‐yl tripeptide (D ‐(Ad‐1‐yl)Gly‐L ‐Ala‐D ‐isoGln) was prepared to study the effects of mannosylation on adjuvant (immunostimulating) activity. Mannosylated adamant‐1‐yl tripeptide (Man‐OCH2CH(Me)CO‐D ‐(Ad‐1‐yl)Gly‐L ‐Ala‐D ‐isoGln) is a non‐pyrogenic, H2O‐soluble, and non‐toxic compound. Adjuvant activity of mannosylated adamantyl tripeptide was tested in the mouse model with ovalbumin as an antigen and in comparison to the parent tripeptide and peptidoglycan monomer (PGM, β‐D ‐GlcNAc‐(1→4)‐D ‐MurNAc‐L ‐Ala‐D ‐isoGln‐mesoDAP(εNH2)‐D ‐Ala‐D ‐Ala), a well‐known effective adjuvant. The mannosylation of adamantyl tripeptide caused the amplification of its immunostimulating activity in such a way that it was comparable to that of PGM. |
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| Keywords: | Mannose Peptides Adamant‐1‐yl tripeptide Immunostimulating activity Peptidoglycan |
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