Studies of oligosaccharide linkages by simultaneous determination of component aldehydes in dialdehyde fragments as (2,4-dinitrophenyl)hydrazones |
| |
| Authors: | Susumu Honda Kazuaki Kakehi Kiyoshi Takiura |
| |
| Institution: | Faculty of Pharmaceutical Sciences, Kinki University, Kowakae, Higashi-Osaka Japan;Faculty of Pharmaceutical Sciences, Osaka University, Suita Japan |
| |
| Abstract: | The component aldehydes in dialdehyde fragments formed by periodate oxidation of oligosaccharides were converted quantitatively into the corresponding (2,4-dinitrophenyl)hydrazones by the simple procedure of treatment with excess (2,4-dinitrophenyl)hydrazine hydrochloride in 1,2-dimethoxyethane. Then, by chromatographic separation of the hydrazones on a small column of silica gel and subsequent spectrophotometric analysis, it was possible to determine the position of glycosidic substitution in μmolar amounts of various types of glucobioses, oligosaccharides of senega, and some synthetic (1→6)-β-D-gluco-oligosaccharides. |
| |
| Keywords: | To whom all correspondence should be address |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
