In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements,status and prospects |
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Authors: | Saikat Gantait Jitendriya Panigrahi |
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Affiliation: | 1. All India Coordinated Research Project on Groundnut, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal 741235, India;2. Department of Genetics and Plant Breeding, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, India;3. Department of Biotechnology, Shri A.N. Patel Post Graduate Institute of Science and Research, Anand, Gujarat 388001, India |
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Abstract: | Adhatoda vasica Nees, belonging to family Acanthaceae, is a well-known medicinal plant. It is endorsed for its pyrroloquinazoline alkaloids and its derivatives, such as vasicine and vasicinone. Germinating A. vasica seeds is a tedious task; on that account, vegetative propagation is the preferred method for its multiplication. For rapid and large-scale multiplication, germplasm conservation as well as secondary metabolites production, in vitro culture of A. vasica was preferred over conventional propagation by several researchers; however, some major applications of this tissue culture technique are still awaiting to undergo extensive research. The present review, for the first time, illustrates all the major achievements associated with in vitro regeneration of A. vasica, reported till date and highlights the future prospects. |
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Keywords: | Artificial seed Medicinal plant Organogenesis Somatic embryogenesis Vasicine Vasicinone 2,4-D 2,4-dichlorophenoxyacetic acid AC Activated charcoal B5 B5 medium, or Gamborg’s medium BA IAA indole-3-acetic acid IBA indole-3-butyric acid Kn Kinetin or 6-furfurylaminopurine MS Murashige and Skoog NAA α-napthalene acetic acid PGR plant growth regulator SH Schenk and Hildebrandt TDZ thidiazuron |
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