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Chemotaxonomic and Micromorphological Traits of Satureja montana L. and S. subspicata Vis. (Lamiaceae)
Authors:Valerija Dunkić  Dario Kremer  Ivna Dragojević Müller  Edith Stabentheiner  Sunčica Kuzmić  Renata Jurišić Grubešić  Lovorka Vujić  Ivan Kosalec  Marko Randić  Siniša Srečec  Nada Bezić
Affiliation:1. Faculty of Sciences and Mathematics, University of Split, Teslina 12, HR‐21000 Split;2. Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova?i?a 1, HR‐10000 Zagreb, (phone: +385?1?4619422;3. fax: +385?1?6394400);4. Department of Ecology and Water Protection, Water Supply and Drainage, Pata?i?kina 1b, HR‐10000 Zagreb;5. Institute of Plant Sciences, Karl‐Franzens University, Schubertstrasse 51, A‐8010 Graz;6. Forensic Science Centre ‘Ivan Vu?eti?', Ilica 335, HR‐10000 Zagreb;7. Public institution ‘Priroda', Grivica 4, HR‐51000 Rijeka;8. Kri?evci College of Agriculture, M. Demerca 1, HR‐48260 Kri?evci
Abstract:Satureja montana and S. subspicata are used as spice, pepper substitute, for preparing tea, juice, and as a medicine. Fourteen populations (seven per species) of Satureja montana L. and S. subspicata Vis . growing in Croatia were examined to determine the chemical composition of the essential oil (analyzed by GC‐FID and GC/MS), the content of macroelements (Na, K, Ca, Mg) and trace elements (B, Fe, Cu, Mn, Zn, Al, Pb, Cr, Cd, Ni, Hg, As) analyzed by ICP‐AES, antioxidant compounds (analyzed by UV/VIS spectrophotometer), and the types and distribution of trichomes (analyzed by scanning electron microscopy). The main constituents of the essential oil were carvacrol and thymol in S. montana (all populations belong to one phenol chemotype), while α‐eudesmol, β‐eudesmol, and spathulenol dominated in S. subspicata (three chemotypes could be distinguished). Both species possess considerably higher quantities of Ca and Mg, and moderate concentrations of K and Na, while Hg and As levels were below the limit of quantification. Non‐glandular trichomes, peltate trichomes, and three types of capitate trichomes were observed on leaves, stem, calyx, and corolla.
Keywords:Satureja species  Essential oils  Trace elements  Phenolic compounds  Trichomes  Pollen  Principal‐component analysis (PCA)  Chemotaxonomy
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