Determination of the linkages in some methylated,sialic acid-containing,meningococcal polysaccharides by mass spectrometry |
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Authors: | Apurba K Bhattacharjee Harold J Jennings |
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Institution: | Division of Biological Sciences, National Research Council of Canada, Ottawa KIA OR6 Canada |
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Abstract: | The application of gas-liquid chromatography-mass spectrometric (g.l.c.-m.s.) analysis to a number of sialic acid-containing polysaccharides of meningococcal origin has been studied. Methylation of these polysaccharides by the Hakomori conditions resulted in both O- and N-methylation. Methanolysis of the methylated polysaccharides from serogroup C (2→9)-linked], colominic acid (2→8)-linked], and serogroups Y and W-135 both (1→4)-linked], yielded the respective 4,7,8,4,7,9-, and 7,8,9-tri-O-methyl derivatives of methyl N-acetyl-N-methyl-β-D-neuraminate methyl glycoside. As model compounds, methyl N-acetyl-4,7,8,9-tetra-O-methyl-α-D-neuraminate methyl glycoside and its N-methyl derivative were also synthesized. All of the methylated derivatives could be identified on the basis of their typical fragmentation-patterns, indicating that this method is applicable to the determination of the position of linkages to sialic acid residues in biopolymers. |
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