Bacterial β‐Aminopeptidases: Structural Insights and Applications for Biocatalysis |
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Authors: | Hans‐Peter E Kohler |
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Institution: | 1. EAWAG, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Microbiology, überlandstrasse 133, CH‐8600 Dübendorf (phone: +41?58?765?5521;2. fax: +41?58?765?5547) |
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Abstract: | β‐Aminopeptidases comprise a class of enzymes with functional and structural similarities. All members of the β‐aminopeptidases described to date were isolated from bacterial sources. Uniquely, they catalyze the hydrolysis of β3‐ and/or β2‐amino acid residues from amides and peptides that are otherwise considered proteolytically stable. Due to this unusual reactivity with β‐peptide substrates, β‐aminopeptidases have potential to be used as biocatalysts for β‐peptide synthesis and for the resolution of enantiomerically pure β‐amino acids from racemic substrate mixtures. β‐Aminopeptidases are formed from an inactive precursor by posttranslational autoproteolytic cleavage, exposing the catalytic nucleophile at the N‐terminus of the newly formed β‐polypeptide chain. Such an activation step is a characteristic trait of enzymes of the N‐terminal nucleophile (Ntn) hydrolase superfamily. However, classical Ntn hydrolases and β‐aminopeptidases differ by the fold of their catalytic cores and are hence likely to originate from distinct evolutionary ancestors. In this contribution, we review the existing literature on β‐aminopeptidases, including biochemical and functional studies, as well as structural investigations that recently allowed insights into the catalytic mechanisms of precursor processing and β‐peptide conversion. |
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Keywords: | β ‐Aminopeptidase Ntn Hydrolase Amino acids Peptides β ‐Lactam antibiotics Antibiotics DmpA/OAT Superfamily Biocatalysis |
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