Effects of trace metals on mouse B16 melanoma cells in culture |
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Authors: | Eliane Bahbouth Brigitte Siwek Marie-Claire De Pauw-Gillet Enrico Sabbioni Roger Bassleer |
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Institution: | (1) Environment Institute, CEC, JRC Ispra Site, I-21020 Ispra (Varese), Italy;(2) Department of Histology and Cytology, University of Liège, Rue de Pitteurs 20, B-4020 Liège, Belgium |
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Abstract: | The effects of fourteen metal ions (As3+, As5+, Cd2+, Co2+, Cr3+, Cr6+, Hg2+, Li+, Mg2+, Mn2+, Ni2+, Se4+, V5+, VO2+) on the proliferation and differentiation in mouse B16 melanoma cells cultivated in vitro were analyzed. Cell number assays,
melanin, and protein measurements, a 3(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide reduction test (MTT survival
test), and a clonal growth assay were performed. At 10−4
M, metal ions such as As3+, As5+, Cd2+, Cr6+, Se4+, V5+, VO2+, and, to a minor extent, Li+, Hg2+, and Co2+ significantly reduced the number of the B16 melanoma cells. For the same molar concentration, the order of the levels of
cell toxicity of the metal compounds to B16 cells as measured by the MTT test was as follows: Hg2+>Cr6+=Cd2+>As3+, As5+>V5+, VO2+>Se4+=Ni2+=Co2+=Li+. An increased synthesis of melanin in B16 cells was noted after incubation with Co2+, Ni2+, Cd2+, and Li+, whereas Se4+ had, on the contrary, an inhibiting effect on melanogenesis. |
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Keywords: | Trace metals in vitro effects of B16 melanoma cells cell differentiation cytotoxicity protein synthesis melanin |
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