Chloramphenicol resistance in Streptomyces coelicolor A3(2): Possible involvement of a transposable element |
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Authors: | Giuseppe Sermonti Anna Petris Mariarita Micheli and Luisa Lanfaloni |
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Institution: | (1) Institute of Histology and Embryology, The University, Perugia, Italy;(2) Institute of Histology and Embryology, Via Elce di Sotto, I-06100 Perugia, Italy |
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Abstract: | Summary The transfer of a Chl element, causing resistance to chloramphenicol in Streptomyces coelicolor A3(2), was studied in NF x SCP1– superfertile crosses. When the Chl element is on the donor side (NF) its transfer to the recombinant cells was virtually total as if the element acted as a second concomitant transfer origin. When the Chl element was on the recipient side (SCP1–) it was never displaced by the immigrant chromosome even when the region facing chl
+ was selected for. A fraction of the original Chl– mutants presented a requirement for arginine (ArgB–). A Chl– mutant gave rise spontaneously to ArgB– derivatives at high frequency. The same ArgB– requirement come out at high frequency among Chl– derivatives from a cross NFChl– x SCP1–Chl+ in which neither parent required arginine or produced spontaneously arginineless derivatives. It is suggested that the Chl element is a transposable element (Tn) presumably associated with insertion sequences (IS). The insertional inactivation of the Chl element may be accompanied or followed by a deletion in the adjacent ArgB gene. |
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