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Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT
Authors:Cynthia L Pon  Martin Brombach  Sabine Thamm and Claudio O Gualerzi
Institution:(1) Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, D-1000 Berlin 33;(2) Laboratory of Genetics, DBC, University of Camerino, I-62032 Camerino, Italy
Abstract:Summary Using two synthetic deoxyribonucleotide probes encoding segments of the primary structure of initiation factor IF3 from Bacillus stearothermophilus, we identified and cloned a segment of DNA which carries the infC gene. As in Escherichia coli, the infC gene begins with the unusual initiation triplet AUU, and is followed by the structural genes for ribosomal proteins L35 and L20 (rpmI and rplT, respectively).
Keywords:Gene cloning  Protein synthesis  Initiation factor  Ribosomal protein  Thermophile
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