Hydrogen peroxide mediates reperfusion injury in the isolated rat heart |
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| Authors: | James M Brown Lance S Terada Michael A Grosso Glenn J Whitman Stephen E Velasco Anita Patt Alden H Harken John E Repine |
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| Institution: | (1) Departments of Surgery and Medicine, and the Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver, Colorado, USA |
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| Abstract: | In an isolated, normothermic rat heart model (Langendorff, 37 °C), dimethylthiourea (DMTU) infusion only during reperfusion reduced both injury and measurable hydrogen peroxide (H2O2) concentrations after global ischemia. Cardiac function was assessed by measurement of ventricular developed pressure (DP). H2O2 was assessed using H2O2 dependent aminotriazole inactivation of myocardial catalase. Depletion of xanthine oxidase by two methods (tungsten or allopurinol inhibition) also improved recovery of function and H2O2 production. The results indicate that XO derived H2O2 contributes to myocardial reperfusion injury. |
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| Keywords: | eschemia-reperfusion global ischemia xanthine oxidase depletion allopurinol |
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