Reaction on alternating GC oligonucleotide templates |
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Authors: | K-m. E. Ng L. E. Orgel |
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Affiliation: | (1) The Salk Institute for Biological Studies, PO Box 85800, 92138 San Diego, California, USA |
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Abstract: | Summary The condensation reactions of activated nucleotides, ImpN or 2-MeImpN, with the selfcomplementary ribo-octanucleotide GCGCGCGC or with the partially self-complementary heptanucleotide GCGCGCG were studied. The templatedirected reaction of 2-meImpC with the heptamer yields the 3–5 octamer as the main product. All other reactions yield 2–5-linked octamers and pyrophosphates as major products. Surprisingly, 2-MeImpG facilitates the reaction of 2-MeImpC with the heptamer.Procedures for the analysis by gel electrophoresis of the oligomeric products obtained in reactions of this kind are described. |
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Keywords: | Template-directed condensation Oligonucleotides Activated nucleotides Gel electrophoresis |
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