Kinetics of product formation in batch and continuous culture of Clostridium barkeri |
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Authors: | Lena Häggström |
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Institution: | (1) Department of Biochemistry and Biotechnology, Royal Institute of Technology, S-10044 Stockholm, Sweden |
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Abstract: | Summary The main fermentation end products in batch culture (unlimited glucose supply) of Clostridium barkeri were butyrate and lactate. The specific rate of butyrate production was linearly proportional to the growth rate while the specific rate of lactate production increased at low growth rates. In a glucose limited chemostat culture butyrate production was partly growth associated while acetate and lactate production was growth associated. Lactate was, however, only produced at high dilution rates. By varying the glucose concentration in the inflowing medium it was shown that lactate production was stimulated by a high feeding rate of the carbon source. These results are discussed in view of the fructose-1,6-diphosphate dependent lactate dehydrogenase activity in many other organisms. |
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