ELF磁场对3T3细胞生长的影响及其机理的探讨

用细胞分析成像光盘记录系统测量了3T3细胞在某些ELF磁场和温度条件下生长周期的变化.实验结果表明,只有某些频率的ELF磁场才对细胞生长产生影响,磁场对细胞生长的影响还与培养细胞的生化环境有关.温度和ELF磁场都能影响细胞的生长.但二者的机理是不一样的,当撤除ELF磁场后,细胞在短时间内(2天以上)继续保持着ELF场对其生长的影响.而细胞生长周期能在短时间内(2天以内)随着温度的变化而变化.温度引起的细胞生长的变化可能与细胞内的各种生长因子、生物离子的活性有关.ELF磁场引起的细胞生长的变化可能与ELF磁场对细胞膜的影响有关,与细胞内细胞生长必不可少的生物离子(如Ca~(2+))的浓度有关.

THE EFFECT AND MECHANISM OF MAGNETIC FIELDS ON 3T3 CELL PROLIFERATION

The cell cycles of MH 3T3 cell in ELF magnetic fields and at different temperatures aere measured and compared by using Automated Cinematographic Records System. The experiment results showed: (1) The cell cycles changed distinctly in some ELF magnetic fields tuned on some ion cyclotron resonance condition. (2) Both temperature and ELF magnetic fields could affect the cell cycles. (3) The cell proliferation could be changed quickly with a change of temperature. but couldn't be recovered quickly (in two days time) after ELF magnetic fields were removed. This result implied that there is a difference between temperature effect and ELF magnetic effect.