Purification and characterization of uracil phosphoribosyltransferase from Crithidia luciliae |
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| Authors: | T Asai C S Lee A Chandler W J O'Sullivan |
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| Institution: | School of Biochemistry, University of N.S.W., Kensington, Australia. |
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| Abstract: | 1. Uracil phosphoribosyltransferase (UPRTase) was purified 370-fold from the protozoan parasite, Crithidia luciliae. 2. The enzyme was a dimer of mol. wt 80 000 and was highly specific for uracil. 3. GTP, which is an activator of UPRTase from E. coli had a slight inhibitory effect on the parasite enzyme. 4. The C. luciliae UPRTase demonstrated a broad specificity for activating divalent metal ions. |
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