Effect of molybdenum on the efficiency of tetrathionate utilisation by chemostat cultures ofSulfolobus BC |
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Authors: | Buckingham J. A. Stanbury P. F. LeRoux N. W. |
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Affiliation: | (1) Department of Biological Sciences, Hatfield Polytechnic, AL10 9AB Hatfield, Herts., England;(2) Biotechnology and Separations Division, Department of Trade and Industry, Warren Spring Laboratory, SG1 2BX Stevenage, Herts., England |
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Abstract: | Summary Continuous cultures ofSulfolobus BC oxidising tetrathionate were found not to be tetrathionate limited, but molybdenum limited. Incorporation of only 1.3M Na2MoO4 into the medium of autotrophically grown, continuous cultures ofSulfolobus BC oxidising 15mM tetrathionate at a dilution rate of 0.029h–1 increased the steady state biomass from absorbance (660nm) 0.160 to 0.315. The biomass yield gave a corresponding increase from 5.93±0.84 to 11.52±0.68 g dry weight mol–1 tetrathionate oxidised. |
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