Purification and characterization of a novel 7-kDa non-specific lipid transfer protein-2 from rice (Oryza sativa) |
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| Authors: | Liu Yaw-Jen Samuel Dharmaraj Lin Chi-Hung Lyu Ping-Chiang |
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| Affiliation: | Department of Life Sciences, National Tsing Hua University, Hsinchu, Taiwan, ROC. |
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| Abstract: | A novel 7-kDa non-specific lipid transfer protein-2 (nsLTP2) has been isolated from rice (Oryza sativa) seeds. In contrast to nsLTP1s, few nsLTP2s have been purified and characterized. Complete amino acid sequence of rice nsLTP2 was determined by N-terminal Edman degradation of the intact protein as well as the peptide fragments resulted from trypsin digestions. Rice nsLTP2 consists of 69 amino acid residues with eight conserved cysteines forming four disulfide bonds. The secondary structure of rice nsLTP2 is predominantly alpha-helical as determined by circular dichroism spectroscopy. Cysteine pairings of nsLTP2 have one miss match at Cys(35)-X-Cys(37) motif compared to nsLTP1. Primary structure analysis of various plant nsLTP2s revealed an interesting conservation of sequence features among nsLTP2 family. |
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| Keywords: | Novel protein Plant nsLTP2 Proteolytic digestion Trypsin Thermolysin Protein sequencing Disulfide bond pattern Lipid transfer All α-helical protein |
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