Isolation and partial characterization of normal and defective bacteriophages of gram-negative hydrogen bacteria |
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Authors: | Auling Georg Mayer Frank Schlegel Hans Günter |
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Affiliation: | (1) Institut für Mikrobiologie der Universität Göttingen und Institut für Mikrobiologie der Geselischaft für Strahlen- und Umweltforschung mbH, München, Grisebachstr. 8, D-3400 Göttingen, Germany |
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Abstract: | Widespread defective lysogeny was detected in Alcaligenes eutrophus by electron microscopic analysis of cultures. Mitomycin C treatment of the cultures resulted in the production of defective (inco-) particles. Polysheaths were produced both with and without induction.With the simultaneous isolation technique six phages were isolated for hydrogen-oxidizing strains of the new species Pseudomonas pseudoflava. The phages were able to replicate under autotrophic conditions and were found to have a very restricted host range. Electron microscopic analysis allowed classification into two structural groups. Group I contained phages with contractile tails; group II contained phages with flexible, noncontractile tails. All but one (gb) of the new phages were shown to be temperate by isolation of lysogens and induction with mitomycin C.Non-Standard Abbreviations OD optical density - SM-medium standard mineral medium - KS-medium Kaserer medium - NB nutrient broth - p.f.u. plaque forming unit - c.f.u. colony forming unit - e.o.p. efficiency of plating - Do-phage buffer Dopatka-phage buffer - PTA phosphotungstic acid |
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Keywords: | Defective lysogeny Alcaligenes eutrophus Simultaneous isolation technique Temperate bacteriophages Pseudomonas pseudoflava Biological characterization Electron microscopy |
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