| Interaction between a Nanovirus-like Component and the Tobacco Curly Shoot Virus／Satellite Complex |
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| 作者姓名： | Wu PJ Zhou XP |
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| 作者单位： | InstituteofBiotechnology,ZhejiangUniversity,Hangzhou310029,China |
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| 基金项目： | This work was supported by the grants from the National Outstanding Youth Foundation(No.30125032),the National NaturalScience Foundation of China(No.30370927) |
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| 摘 要： | The biological role of DNA1, a nanovirus-like component shown to be associated with the begomovirus/satellite complex, has not yet been identified. Here, we demonstrated that DNA1 of Tobacco curly shoot virus isolate Y35 (TbCSV-Y35) attenuated leaf-curling symptoms induced by TbCSV-Y35 or TbCSV-Y35 plus Y35 DNAβ in the early stage of symptom development and induced leaf cluster at a later stage of symptom development in Nicotiana benthamiana plants. The leaf disc assay demonstrated that TbCSV-Y35 DNA1 replicated autonomously. Southern blot analysis revealed that TbCSV-Y35 DNA1 reduced viral DNA accumulation. Viral DNA accumulation was not reduced when plants were co-inoculated with TbCSV-Y35 DNAβ, but the TbCSV-Y35 DNAβ level was dramatically reduced in the presence of TbCSV-Y35 DNA1. To determine whether the interaction between TbCSV/satellite complex and DNA1 had isolate specificity, DNA1 of TbCSV isolate Y132 was cloned and sequenced. It was found to have 75% nucleotide sequence identity with TbCSV-Y35 DNA1. Infectivity tests showed that TbCSV-Y132 DNA1 had no effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAβ in N. benthamiana plants, although Y 132 DNA1 could replicate in these plants.
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| 关 键 词： | 烟草枝条卷曲病毒 纳米病毒 DNA1 DNAβ 症状 基因克隆 交互作用 |
Interaction between a nanovirus-like component and the Tobacco curly shoot virus/satellite complex |
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| Wu PJ,Zhou XP.Interaction between a nanovirus-like component and the Tobacco curly shoot virus/satellite complex[J].Acta Biochimica et Biophysica Sinica,2005,37(1):25-31. |
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| Authors: | Wu Pei-Jun Zhou Xue-Ping |
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| Institution: | Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China. |
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| Abstract: | The biological role of DNA1, a nanovirus-like component shown to be associated with the begomovirus/satellite complex, has not yet been identified. Here, we demonstrated that DNA1 of Tobacco curly shoot virus isolate Y35 (TbCSV-Y35) attenuated leaf-curling symptoms induced by TbCSV-Y35 or TbCSV-Y35 plus Y35 DNAbeta in the early stage of symptom development and induced leaf cluster at a later stage of symptom development in Nicotiana benthamiana plants. The leaf disc assay demonstrated that TbCSV-Y35 DNA1 replicated autonomously. Southern blot analysis revealed that TbCSV-Y35 DNA1 reduced viral DNA accumulation. Viral DNA accumulation was not reduced when plants were co-inoculated with TbCSV-Y35 DNAbeta, but the TbCSV-Y35 DNAbeta level was dramatically reduced in the presence of TbCSV-Y35 DNA1. To determine whether the interaction between TbCSV/satellite complex and DNA1 had isolate specificity, DNA1 of TbCSV isolate Y132 was cloned and sequenced. It was found to have 75% nucleotide sequence identity with TbCSV-Y35 DNA1. Infectivity tests showed that TbCSV-Y132 DNA1 had no effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAbeta in N. benthamiana plants, although Y132 DNA1 could replicate in these plants. |
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| Keywords: | Tobacco curly shoot virus DNA1 DNAβ symptom replication interaction |
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