Hydrogen production by Rhodobacter sphaeroides strain O.U.001 using spent media of Enterobacter cloacae strain DM11 |
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Authors: | Kaushik Nath Anish Kumar Debabrata Das |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India |
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Abstract: | Combined dark and photo-fermentation was carried out to study the feasibility of biological hydrogen production. In dark fermentation,
hydrogen was produced by Enterobacter cloacae strain DM11 using glucose as substrate. This was followed by a photo-fermentation process. Here, the spent medium from the
dark process (containing unconverted metabolites, mainly acetic acid etc.) underwent photo-fermentation by Rhodobacter sphaeroides strain O.U.001 in a column photo-bioreactor. This combination could achieve higher yields of hydrogen by complete utilization
of the chemical energy stored in the substrate. Dark fermentation was studied in terms of several process parameters, such
as initial substrate concentration, initial pH of the medium and temperature, to establish favorable conditions for maximum
hydrogen production. Also, the effects of the threshold concentration of acetic acid, light intensity and the presence of
additional nitrogen sources in the spent effluent on the amount of hydrogen produced during photo-fermentation were investigated.
The light conversion efficiency of hydrogen was found to be inversely proportional to the incident light intensity. In a batch
system, the yield of hydrogen in the dark fermentation was about 1.86 mol H2 mol−1 glucose; and the yield in the photo-fermentation was about 1.5–1.72 mol H2 mol−1 acetic acid. The overall yield of hydrogen in the combined process, considering glucose as the preliminary substrate, was
found to be higher than that in a single process. |
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