马立克氏病病毒和火鸡疱疹病毒在鸡胚细胞培养上生长特性的比较

COMPARISON OF GROWTH CHARACTERISTICS BETWEEN MAREK'S DISEASE VIRUS AND HERPESVIRUS OF TURKEY IN CHICK EMBRYONIC CELL CULTURE

Infected with different strains of MDV, variation of virus plaque was observed in chick embryonic cell culture. The shape of the plaques varied in accordance with the virulence of the MDV strains. The more virulent strains produced smaller plaques. The ascending order of plaque size in cell culture is as follows: MDV-Md5,MDV-GA, MDV-SB1, MDV Mdll/75c and HVT-Fcl26. The latent period of the virus is related to the amount of virus particles inoculated, the more virus inoculated, the earlier CPE appeared. By measuring the growth rate of the virus and observing with the electron microscope ( EM ) , probably secondary infection was found in cells infected with HVT-Fc126,but not with MDV-GA. From the results of EM observation, we suggest that the infection mode of MDV-GA is to move from one nucleus to another while forming a syncytium and the infection mode of HVT-Fcl26 includes both secondary infection and syncytial formation.