| 一株转化淀粉或麦芽寡糖生成海藻糖的菌株D-97鉴定 |
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| 引用本文： | 荣绍丰, 徐骥, 张海平, 杨静, 段作营, 毛忠贵,.一株转化淀粉或麦芽寡糖生成海藻糖的菌株D-97鉴定[J].微生物学通报,2003,30(2):57-61. |
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| 作者姓名： | 荣绍丰 徐骥 张海平 杨静 段作营 毛忠贵 |
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| 作者单位： | 江南大学生物工程学院无锡214036 |
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| 摘 要： | 由东北大田采集的土样中筛选到菌株D-97，该菌株胞内酶可以利用淀粉或麦芽寡糖合成海藻糖。通过生理、形态、结构特征分析及16SrDNA基因全序列与参比菌株的序列比较，菌株D-97与食尼古丁节杆菌的16SrDNA序列同源性高达97．98％，故将该菌株命名为食尼古丁节杆菌D-97（Arthrobacter nicotinovorus D-97)。我们还将D-97菌株与日本林原公司的海藻糖生产苗——节杆菌Q36的有关生理生化特征进行了比较。
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| 关 键 词： | 海藻糖 菌株鉴定 淀粉 麦芽寡糖 食尼古丁节杆菌D－97 生成菌 |
| 文章编号： | 0253-2654(2003)02-0057-05 |
| 修稿时间： | 2002年4月28日 |
IDENTIFICATION OF BACTERIUM D-97 PRODUCING TREHALOSE FROM STARCH OR MALTOOLIGOSACCHRIRIDES |
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| RONG Shao,Feng,XU Ji,ZHANG Hai,Ping,YANG Jing,DUAN Zuo,Ying,MAO Zhong,Gui.IDENTIFICATION OF BACTERIUM D-97 PRODUCING TREHALOSE FROM STARCH OR MALTOOLIGOSACCHRIRIDES[J].Microbiology,2003,30(2):57-61. |
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| Authors: | RONG Shao Feng XU Ji ZHANG Hai Ping YANG Jing DUAN Zuo Ying MAO Zhong Gui |
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| Abstract: | A bacterium D 97 whose enducellular enzymes can produce trehalose from starch or maltooligosaccharides was isolated from Datian in Northeast of China The morphological, Cultural and Physiological Characteristics of trehalose prodicing bacterium D 97 were described in this paper There are galactose and lysine in its cell wall but no diaminopimelic acid (DAP) arabinose The content of G+C mol% is 61 3 The full lenth of 16s rDNA (770bp) has been tested and compared with the type strains of all known Arthrobacter sp in Genebank, the results showed the similarity values of 16s rDNA sequence between bacterium D 97 and Arthrobacter nicotinovorus were 97 98%, therefore bacterium D 97 was nameed Arthrobacter nicotinovorus D 97 In this paper we also carried out some comparisons of Physiological Characteristics between Arthrobacter nicotinovorus D 97 and Arthrobacter sp Q36 |
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| Keywords: | Trehalose Strain identification Arthrobacter nicotinovorus D 97 |
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