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甲胎蛋白在体外对人肝癌细胞生长的影响
引用本文:王兴旺,谢弘. 甲胎蛋白在体外对人肝癌细胞生长的影响[J]. 分子细胞生物学报, 1999, 0(1)
作者姓名:王兴旺  谢弘
作者单位:中国科学院上海细胞生物学研究所,中国科学院上海细胞生物学研究所 上海 200031,上海 200031
摘    要:本文用MTT比色法观察了甲胎蛋白(AFP)在体外对人肝癌细胞生长的影响。结果表明,AFP能促进SMMC-7721人肝癌细胞的生长。当AFP与AFP抗体合用时,AFP抗体能减弱AFP对SMMC-7721细胞生长的促进作用;AFP抗体单用对此种细胞的生长亦有抑制作用。另一方面,在相同的实验条件下,AFP和AFP抗体对HL-60人白血病细胞的生长无明显影响;提示AFP的促生长作用具有一定的肿瘤细胞特异性,并非一种蛋白质对培养细胞的非特异性营养作用。此外,AFP亦能促进MCF-7人乳腺癌细胞的生长,AFP抗体对此种细胞的生长有抑制作用。由于MCF-7细胞存在功能性AFP受体,也能合成和分泌AFP。这就提示,人肝癌细胞中的AFP很可能与其受体特异性结合,产生促生长效应。确切机制尚待进一步阐明。

关 键 词:甲胎蛋白  甲胎蛋白抗体  肝癌细胞系  乳腺癌细胞系  白血病细胞系  细胞生长  MTT比色法

EFFECT OF ALPHA-FETOPROTEIN ON THE GROWTH OF HUMAN HEPATOMA CELLS IN VITRO
WANG Xing Wang XIE Hong. EFFECT OF ALPHA-FETOPROTEIN ON THE GROWTH OF HUMAN HEPATOMA CELLS IN VITRO[J]. Journal of Molecular Cell Biology, 1999, 0(1)
Authors:WANG Xing Wang XIE Hong
Abstract:The stimulatory activity of human alpha-fe-toprotein ( AFP ) on the growth of mouse hep-atoma-22 cells had been reported in our previous paper. The present work aimed at further investigation of the effect of AFP on human hepatoma cell growth by MTT colorimetric assay. The results showed that AFP could stimulate the growth of SMMC-7721 human hepatoma cells in vitro. The present results also showed that the stimulatory effect of AFP on the growth of SMMC-7721 cells was decreased by the anti-serum of AFP. The anti-AFP antibody alone could suppress the growth of SMMC-7721 cells. On the other hand, AFP and anti-AFP antibodyhad no effect on the growth of HL-60 human leukemia cells, indicating that the tumor cell growth stimulating effect of AFP was not simply due to non-specific addition of exogenous protein and this effect of AFP showed strict tumor cell specificity. In addition, MCF-7 human breast cancer cell growth was also promoted by AFP and inhibited by anti-AFP antibody. Because AFP cell-surface receptors have been detected in MCF-7 breast cancer cells, and AFP could also be produced and secreted by MCF-7 cells, the possibility may be considered: AFP may bind with its receptors on tumor cell membrane for the purposes of growth stimulation.
Keywords:AIpha-fetoprotein( AFP ). Anti-AFP antibody. Hepatoma cells. Breast cancer cells. Leukemia cells. Cell growth. MTT colorimetric assay  
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