Sequence variability in the FUM1 gene of Fusarium verticillioides strains |
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Authors: | da Silva Valéria Nascimento de Araujo Jansen Durigon Edison Luiz Corrêa Benedito |
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Affiliation: | Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de S?o Paulo, Av Prof. Lineu Prestes, 1374, Cidade Universitária, CEP 05508-900 S?o Paulo, SP, Brazil. valenas@ig.com.br |
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Abstract: | Fumonisins are mycotoxins, produced mainly by Fusarium verticillioides, that are potentially carcinogenic to humans and toxic to animals. Synthesis of these toxins is directed by a cluster of 15 genes, among which FUM1 is the largest; it encodes a polyketide synthase. This enzyme probably catalyzes the synthesis of a polyketide that forms a large portion of the fumonisin structure. In this study, 27 strains possessing the FUM1 gene, as determined by polymerase chain reaction, were analyzed. A portion of the FUM1 gene was amplified and sequenced from 6 of 27 Brazilian strains isolated from corn and sorghum. The sequence similarity for the six F. verticillioides strains was almost 100%. |
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