Capacity assessment of Myzus persicae,Aphis gossypii and Aphis spiraecola (Hemiptera: Aphididae) to acquire and retain PVYNTN in Tunisia |
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Authors: | Sonia Boukhris-Bouhachem Ibtissem Ben Fekih Sabrine Nahdi Rebha Souissi |
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Affiliation: | 1.Plant Protection Laboratory of National Institute of Agricultural Research of Tunisia,Ariana,Tunisia;2.Dry Land Farming and Oasis Cropping Laboratory,Arid Land Institute,El Fjè-Medenine,Tunisia |
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Abstract: | The study was carried out to investigate the ability of three aphids, Myzus persicae, Aphis gossypii and Aphis spiraecola, to acquire and retain the Potato Virus Y (PVY) isolate, PVYNTN. Tobacco plants, Nicotiana tabacum var. Xanthi, were used as test plant for the virus inoculation and aphid acquisition. The serological test double-antibody sandwich enzyme-linked immunosorbent assay was applied for virus detection on the test plants and aphids. Furthermore, virus retention by aphids was also assessed using a monoclonal anti-PVYN. Although a duration of 2 min was enough for the virus acquisition, the three tested aphids showed different capacities to retain PVYNTN. The retention of PVYNTN was 3 h for M. persicae and A. spiraecola, and 2 h for A. gossypii. This study provides basic information of the virus retention by potato-colonizing aphid species, which may increase our understanding of PVY epidemiology in Tunisia. |
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