Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts |
| |
Authors: | Kuroda Hiroshi Suzuki Haruka Kusumegi Takahiro Hirose Tetsuro Yukawa Yasushi Sugiura Masahiro |
| |
Affiliation: | Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya, Japan. |
| |
Abstract: | The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3' end of 16S rRNA facilitates translation initiation from the GUG. |
| |
Keywords: | GUG codon - In vitro - psbC - Shine-Dalgarno sequence - Tobacco - Translation |
本文献已被 PubMed Oxford 等数据库收录! |
|