Immobilization of Saccharomyces cerevisiae cystathionine gamma-lyase and application of the product to cystathionine synthesis |
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| Authors: | Yamagata Shuzo Akamatsu Tsuyoshi Iwama Tomonori |
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| Institution: | Department of Applied Life Sciences, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. |
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| Abstract: | Cystathionine gamma-lyase of Saccharomyces cerevisiae was immobilized to aminohexyl-Sepharose through the cofactor pyridoxal 5'-phosphate and was characterized with respect to its cystathionine gamma-synthase activity. The immobilized product was so stable that it repeatedly catalyzed as many as five cycles of the reaction without losing activity. |
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