Some properties of vacuoles isolated from Neurospora crassa slime variant |
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| Authors: | E Martinoia U Heck Th Boller A Wiemken Ph Matile |
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| Institution: | (1) Department of General Botany, Swiss Federal Institute of Technology, Sonneggstr. 5, CH-8092 Zürich, Switzerland |
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| Abstract: | A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total  -amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.Non-Standard Abbreviation DEAE-dextran
diethylaminoethyl-dextran |
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| Keywords: | Neurospora crassa Slime variant Vacuoles Isolation Hydrolases Arginine permease |
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