RAPID TEST FOR DIAGNOSIS OF ENTEROHEMORRHAGIC E. COLI O157:H7 IN HUMAN STOOL SAMPLES |
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Authors: | SHI-FA DING YI-XUE ZHAO LINDA LIU SHAHIN HATAMI PETER LEA |
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Institution: | umediklnc. 36 Meteor Drive Toronto, Ontario Canada M9W 1A4 |
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Abstract: | A new rapid test platform for the direct detection of E. coli O157:H7 in stool samples of infected patients was compared with the current standard methods. The new test, DIAPRO FAST-Q®, used biochip ICEflo ®technology that provided results within 20 min. Twenty-one stool samples from patients infected with E. coli O157:H7 or of unknown status were studied. Using the DIAPRO FAST-Q® method, within 20 min, we confirmed positive results for the seven known E. coli O157:H7 samples. While the standard culture method gave rise to only four positives in the remaining 14 unknown samples, the DIAPRO FAST-Q® detected E. coli O157:H7 in eight of these samples, which was confirmed subsequently by broth enrichment culture method. |
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